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CCDC51 Polyclonal antibody

CCDC51 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20465-1-AP

Synonyms

CCDC51, MITOK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC51 Polyclonal antibody (20465-1-AP)

    WB analysis using 20465-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20465-1-AP (CCDC51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CCDC51 Polyclonal antibody (20465-1-AP)

    IHC staining of human ovary tumor using 20465-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20465-1-AP (CCDC51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CCDC51 Polyclonal antibody (20465-1-AP)

    IHC staining of human ovary tumor using 20465-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20465-1-AP (CCDC51 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC51 Polyclonal antibody (20465-1-AP)

    IF Staining of HeLa using 20465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20465-1-AP (CCDC51 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20465-1-AP is CCDC51 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC51 fusion protein Ag14301
Full Name coiled-coil domain containing 51
Calculated Molecular Weight 411 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC004341
Gene Symbol CCDC51
Gene ID (NCBI) 79714
RRIDAB_10700001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC51 antibody 20465-1-APDownload protocol
IHC protocol for CCDC51 antibody 20465-1-APDownload protocol
IF protocol for CCDC51 antibody 20465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols