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cyclin I Polyclonal antibody

cyclin I Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16357-1-AP

Synonyms

CCNI, CYC1, cyclin I, CYI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cyclin I Polyclonal antibody (16357-1-AP)

    WB analysis using 16357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using cyclin I Polyclonal antibody (16357-1-AP)

    WB analysis of rat skeletal muscle using 16357-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using cyclin I Polyclonal antibody (16357-1-AP)

    WB analysis of human brain using 16357-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human placenta using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human placenta using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human testis using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human testis using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human brain using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human brain using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human liver using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human liver using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human spleen using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human spleen using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human lung using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human lung using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human ovary using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using cyclin I Polyclonal antibody (16357-1-AP)

    IHC staining of human ovary using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16357-1-AP targets cyclin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cyclin I fusion protein Ag0493
Full Name cyclin I
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC004975
Gene Symbol CCNI
Gene ID (NCBI) 10983
RRIDAB_2275606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for cyclin I antibody 16357-1-APDownload protocol
IHC protocol for cyclin I antibody 16357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols