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CD300A Monoclonal antibody

CD300A Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F9C5

Cat no : 67242-1-Ig

Synonyms

CD300A, CD300a molecule, CLM 8, CMRF 35 H9, CMRF 35H, CMRF35 H, CMRF35 H9, CMRF35 like molecule 8, CMRF35H, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, NK inhibitory receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of THP-1 using 67242-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of K-562 using 67242-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of HL-60 using 67242-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of human spleen using 67242-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of Raji using 67242-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of Jurkat using 67242-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of THP-1 cells using CD300A Monoclonal antibody (67242-1-Ig)

    FC experiment of THP-1 using 67242-1-Ig

    1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD300A (67242-1-Ig, Clone:2F9C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, human spleen tissue, Jurkat cells, K-562 cells, Raji cells
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67242-1-Ig targets CD300A in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD300A fusion protein Ag5776
Full Name CD300a molecule
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC032352
Gene Symbol CD300A
Gene ID (NCBI) 11314
RRIDAB_2882521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CD300A antibody 67242-1-IgDownload protocol
FC protocol for CD300A antibody 67242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols