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CoraLite® Plus 488 Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

30-F11

Cat no : CL488-65087

Synonyms

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD45 (30-F11) (CL488-65087)

    IF Staining of mouse splenocytes using CL488-65087

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD45 (CL488-65087, Clone: 30-F11) at dilution of 1:400.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD45 (30-F11) (CL488-65087)

    FC experiment of mouse splenocytes using CL488-65087

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Mouse CD45 (CL488-65087, Clone: 30-F11) (red) or Control Antibody. Cells were not fixed .

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65087 targets CD45 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse thymus or spleen
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol Ptprc
Gene ID (NCBI) 19264
RRIDAB_2883169
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD45 antibody CL488-65087Download protocol
FC protocol for CL Plus 488 CD45 antibody CL488-65087Download protocol
Standard Protocols
Click here to view our Standard Protocols