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  • Featured Product
  • KD/KO Validated

CENPN Polyclonal antibody

CENPN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16751-1-AP

Synonyms

BM039, C16orf60, CENP N, CENPN, centromere protein N, ICEN32

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CENPN Polyclonal antibody (16751-1-AP)

    WB analysis of MCF-7 using 16751-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16751-1-AP (CENPN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16751-1-AP is CENPN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPN fusion protein Ag10219
Full Name centromere protein N
Calculated Molecular Weight 339 aa, 40 kDa
Observed Molecular Weight 40 kDa, 25-30 kDa
GenBank Accession NumberBC008972
Gene Symbol CENPN
Gene ID (NCBI) 55839
RRIDAB_2878310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.

Protocols

Product Specific Protocols
WB protocol for CENPN antibody 16751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.

Authors - Noritoshi Oka
  • KD Validated
View Article
humanWB,IHC

Cancer Rep (Hoboken)

High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma

Authors - Xiaojie Wang
  • KD Validated
View Article
humanWB

Horm Metab Res

Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway

Authors - Shasha Xu
View Article