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CEP63 Monoclonal antibody

CEP63 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G12B2

Cat no : 66996-1-Ig

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP63 Monoclonal antibody (66996-1-Ig)

    WB analysis using 66996-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP63 Monoclonal antibody (66996-1-Ig)

    IF Staining of hTERT-RPE1 using 66996-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66996-1-Ig targets CEP63 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP63 fusion protein Ag9227
Full Name centrosomal protein 63kDa
Calculated Molecular Weight 703 aa, 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014050
Gene Symbol CEP63
Gene ID (NCBI) 80254
RRIDAB_2882313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

Protocols

Product Specific Protocols
WB protocol for CEP63 antibody 66996-1-IgDownload protocol
IF protocol for CEP63 antibody 66996-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols