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CHST13-Specific Polyclonal antibody

CHST13-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20141-1-AP

Synonyms

C4ST 3, C4ST3, CHST13, CHST13-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    WB analysis of mouse liver using 20141-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20141-1-AP (CHST13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IP experiment of mouse liver using 20141-1-AP

    IP result of anti-CHST13-Specific (IP:20141-1-AP, 4ug; Detection:20141-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IHC staining of human kidney using 20141-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IHC staining of human kidney using 20141-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IHC staining of human hepatocirrhosis using 20141-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IHC staining of human hepatocirrhosis using 20141-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHST13-Specific Polyclonal antibody (20141-1-AP)

    IF Staining of HeLa using 20141-1-AP

    Immunofluorescent analysis of Hela cells, using CHST13 antibody 20141-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20141-1-AP targets CHST13-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name carbohydrate (chondroitin 4) sulfotransferase 13
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_152889
Gene Symbol CHST13
Gene ID (NCBI) 166012
RRIDAB_10667458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST13, also named as C4ST3, belongs to the sulfotransferase 2 family. CHST13 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. CHST13 transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. The antibody is specific to CHST13.

Protocols

Product Specific Protocols
WB protocol for CHST13-Specific antibody 20141-1-APDownload protocol
IHC protocol for CHST13-Specific antibody 20141-1-APDownload protocol
IF protocol for CHST13-Specific antibody 20141-1-APDownload protocol
IP protocol for CHST13-Specific antibody 20141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols