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CPPED1 Polyclonal antibody

CPPED1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20628-1-AP

Synonyms

CPPED1, CSTP1

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using CPPED1 Polyclonal antibody (20628-1-AP)

    WB analysis of SMMC-7721 using 20628-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20628-1-AP (CPPED1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPPED1 Polyclonal antibody (20628-1-AP)

    IHC staining of human liver cancer using 20628-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPPED1 Polyclonal antibody (20628-1-AP)

    IHC staining of human liver cancer using 20628-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20628-1-AP is CPPED1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPPED1 fusion protein Ag14572
Full Name calcineurin-like phosphoesterase domain containing 1
Calculated Molecular Weight 314 aa, 36 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC006289
Gene Symbol CPPED1
Gene ID (NCBI) 55313
RRIDAB_2878711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPPED1, also named as CSTP1, plays an inhibitory role in glucose uptake by adipocytes. It has some isoforms with MW 20 kDa and 28-35 kDa.

Protocols

Product Specific Protocols
WB protocol for CPPED1 antibody 20628-1-APDownload protocol
IHC protocol for CPPED1 antibody 20628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols