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  • KD/KO Validated

CSE1L Polyclonal antibody

CSE1L Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22219-1-AP

Synonyms

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse testis tissue, RAW264.7, MCF-7 cells, Ramos cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman placenta tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22219-1-AP targets CSE1L in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSE1L fusion protein Ag17556
Full Name CSE1 chromosome segregation 1-like (yeast)
Calculated Molecular Weight 971 aa, 110 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC109313
Gene Symbol CSE1L
Gene ID (NCBI) 1434
RRIDAB_10950892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

Protocols

Product Specific Protocols
WB protocol for CSE1L antibody 22219-1-APDownload protocol
IHC protocol for CSE1L antibody 22219-1-APDownload protocol
IP protocol for CSE1L antibody 22219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Nat Commun

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

Authors - Pingting Ying
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
humanWB,IHC

Sci Rep

CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.

Authors - Dong-Dong Cheng
  • KD Validated
humanWB

J Biol Chem

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Authors - Jackson B Trotman
human,mouseWB,IHC,IF

Int J Oncol

Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer.

Authors - Chun-Chao Chang
  • KD Validated
humanWB

J Cancer

CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway.

Authors - Xiao Zhang
  • KD Validated