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CSE1L Monoclonal antibody

CSE1L Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A11

Cat no : 67306-1-Ig

Synonyms

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSE1L Monoclonal antibody (67306-1-Ig)

    WB analysis using 67306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67306-1-Ig (CSE1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSE1L Monoclonal antibody (67306-1-Ig)

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSE1L Monoclonal antibody (67306-1-Ig)

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CSE1L Monoclonal antibody (67306-1-Ig)

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CSE1L Monoclonal antibody (67306-1-Ig)

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells, Jurkat cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67306-1-Ig targets CSE1L in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CSE1L fusion protein Ag29131
Full Name CSE1 chromosome segregation 1-like (yeast)
Calculated Molecular Weight 971 aa, 110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC109313
Gene Symbol CSE1L
Gene ID (NCBI) 1434
RRIDAB_2882568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSE1L antibody 67306-1-IgDownload protocol
IHC protocol for CSE1L antibody 67306-1-IgDownload protocol
IF protocol for CSE1L antibody 67306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols