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CTRL Polyclonal antibody

CTRL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14856-1-AP

Synonyms

chymotrypsin like, Chymotrypsin like protease CTRL 1, CTRL, CTRL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of mouse pancreas using 14856-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of BxPC-3 using 14856-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of mouse pancreas using 14856-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    IHC staining of human pancreas using 14856-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    IHC staining of human pancreas using 14856-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CTRL Polyclonal antibody (14856-1-AP)

    IF Staining of BxPC-3 using 14856-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 14856-1-AP (CTRL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse pancreas tissue, BxPC-3 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14856-1-AP targets CTRL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTRL fusion protein Ag6644
Full Name chymotrypsin-like
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC063475
Gene Symbol CTRL
Gene ID (NCBI) 1506
RRIDAB_10640303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

Protocols

Product Specific Protocols
WB protocol for CTRL antibody 14856-1-APDownload protocol
IHC protocol for CTRL antibody 14856-1-APDownload protocol
IF protocol for CTRL antibody 14856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Thromb Haemost

Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery.

Authors - Susan A Maroney
View Article
mouseWB

Biol Open

Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.

Authors - Timothy L Hostelley
View Article

Oncotarget

The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression.

Authors - Yingying Xie
View Article