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CYP20A1 Monoclonal antibody

CYP20A1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A9E2

Cat no : 67433-1-Ig

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYP20A1 Monoclonal antibody (67433-1-Ig)

    WB analysis using 67433-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IHC staining of human liver cancer using 67433-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IHC staining of human liver cancer using 67433-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IF Staining of HeLa using 67433-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP20A1 Monoclonal antibody (67433-1-Ig)

    IF Staining of HeLa using 67433-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67433-1-Ig targets CYP20A1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP20A1 fusion protein Ag9715
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 49-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2882671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP20A1 antibody 67433-1-IgDownload protocol
IHC protocol for CYP20A1 antibody 67433-1-IgDownload protocol
IF protocol for CYP20A1 antibody 67433-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols