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CYP4F11-Specific Polyclonal antibody

CYP4F11-Specific Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20012-1-AP

Synonyms

CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP4F11-Specific Polyclonal antibody (20012-1-AP)

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP4F11-Specific Polyclonal antibody (20012-1-AP)

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP4F11-Specific Polyclonal antibody (20012-1-AP)

    IF Staining of HepG2 using 20012-1-AP

    Immunofluorescent analysis of HepG2 cells, using CYP4F11 antibody 20012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20012-1-AP targets CYP4F11-Specific in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 4, subfamily F, polypeptide 11
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_001128932
Gene Symbol CYP4F11
Gene ID (NCBI) 57834
RRIDAB_10665946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.

Protocols

Product Specific Protocols
IHC protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
IF protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse

Int Immunopharmacol

Regulation of cytochrome P450 4F11 expression by liver X receptor alpha.

Authors - Tao Zhang
View Article