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DDX43 Polyclonal antibody

DDX43 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17591-1-AP

Synonyms

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDX43 Polyclonal antibody (17591-1-AP)

    WB analysis of HeLa using 17591-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DDX43 Polyclonal antibody (17591-1-AP)

    WB analysis of HepG2 using 17591-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using DDX43 Polyclonal antibody (17591-1-AP)

    WB analysis of A375 using 17591-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DDX43 Polyclonal antibody (17591-1-AP)

    WB analysis of PC-3 using 17591-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX43 Polyclonal antibody (17591-1-AP)

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX43 Polyclonal antibody (17591-1-AP)

    IHC staining of human lung cancer using 17591-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX43 Polyclonal antibody (17591-1-AP)

    IF Staining of HepG2 using 17591-1-AP

    Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17591-1-AP targets DDX43 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX43 fusion protein Ag11484
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC066938
Gene Symbol DDX43
Gene ID (NCBI) 55510
RRIDAB_2090912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DDX43 antibody 17591-1-APDownload protocol
IHC protocol for DDX43 antibody 17591-1-APDownload protocol
IF protocol for DDX43 antibody 17591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients.

Authors - Yasuto Akiyama
View Article