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DDX50 Polyclonal antibody

DDX50 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10358-1-AP

Synonyms

DDX50, DEAD box protein 50, Gu beta, GU2, GUB, Nucleolar protein Gu2, RH II/GuB

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDX50 Polyclonal antibody (10358-1-AP)

    WB analysis of HeLa using 10358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DDX50 Polyclonal antibody (10358-1-AP)

    WB analysis of HeLa using 10358-1-AP

    WB result of DDX50 antibody (10358-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX50 transfected HeLa cells.

  • Western Blot (WB) analysis of Jurkat cells using DDX50 Polyclonal antibody (10358-1-AP)

    WB analysis of Jurkat using 10358-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DDX50 Polyclonal antibody (10358-1-AP)

    IP experiment of HeLa using 10358-1-AP

    IP result of anti-DDX50 (IP:10358-1-AP, 4ug; Detection:10358-1-AP 1:800) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX50 Polyclonal antibody (10358-1-AP)

    IF Staining of HeLa using 10358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX50 antibody (10358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

10358-1-AP targets DDX50 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX50 fusion protein Ag0382
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC000272
Gene Symbol DDX50
Gene ID (NCBI) 79009
RRIDAB_2245834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box polypeptide 50 (DDX50, synonyms: GU2, GUB, MGC3199, RH-II/GuB) enzyme may be involved in ribosomal RNA synthesis or processing.

Protocols

Product Specific Protocols
WB protocol for DDX50 antibody 10358-1-APDownload protocol
IF protocol for DDX50 antibody 10358-1-APDownload protocol
IP protocol for DDX50 antibody 10358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
View Article
humanWB

Arch Virol

DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production.

Authors - Peijun Han
View Article
humanWB

Mol Cell

Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing

Authors - Fardin Aryan
View Article