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CRMP4 Polyclonal antibody

CRMP4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18969-1-AP

Synonyms

CRMP 4, CRMP4, dihydropyrimidinase like 3, DPYSL3, DRP 3, DRP3, LCRMP, ULIP, ULIP 1, ULIP1, Unc 33 like phosphoprotein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CRMP4 Polyclonal antibody (18969-1-AP)

    WB analysis of SH-SY5Y using 18969-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CRMP4 Polyclonal antibody (18969-1-AP)

    WB analysis of SH-SY5Y using 18969-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    WB analysis of mouse thymus using 18969-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    WB analysis of mouse cerebellum using 18969-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CRMP4 Polyclonal antibody (18969-1-AP)

    WB analysis of Jurkat using 18969-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using CRMP4 Polyclonal antibody (18969-1-AP)

    IP experiment of SH-SY5Y using 18969-1-AP

    IP result of anti-CRMP4 (IP:18969-1-AP, 3ug; Detection:18969-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    IHC staining of human liver cancer using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    IHC staining of human liver cancer using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    IHC staining of human kidney using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRMP4 Polyclonal antibody (18969-1-AP)

    IHC staining of human kidney using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CRMP4 Polyclonal antibody (18969-1-AP)

    IF Staining of HepG2 using 18969-1-AP

    Immunofluorescent analysis of HepG2 cells, using DPYSL3 antibody 18969-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inSH-SY5Y cells, Jurkat cells, mouse cerebellum tissue, mouse thymus tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18969-1-AP targets CRMP4 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP4 fusion protein Ag5299
Full Name dihydropyrimidinase-like 3
Calculated Molecular Weight 570 aa, 62 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC039006
Gene Symbol DPYSL3
Gene ID (NCBI) 1809
RRIDAB_10603361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CRMP4 antibody 18969-1-APDownload protocol
IHC protocol for CRMP4 antibody 18969-1-APDownload protocol
IF protocol for CRMP4 antibody 18969-1-APDownload protocol
IP protocol for CRMP4 antibody 18969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.

Authors - Jeremy A Whitson
View Article
ratWB, IP

Biochem Biophys Res Commun

Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina.

Authors - Kazuhiro Tokuda
View Article