Pikachurin Monoclonal antibody
Pikachurin Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1C6D12
Cat no : 66232-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human retina tissue, PC-3 cells |
| Positive IF detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66232-1-Ig targets Pikachurin in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Pikachurin fusion protein Ag6362 |
| Full Name | EGF-like, fibronectin type III and laminin G domains |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 111 kDa, 55-60 kDa |
| GenBank Accession Number | BC033177 |
| Gene Symbol | EGFLAM |
| Gene ID (NCBI) | 133584 |
| RRID | AB_2881623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| IF protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Federico (Verified Customer) (02-18-2020) | The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific.
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