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  • Featured Product
  • KD/KO Validated

ELF2 Polyclonal antibody

ELF2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12499-1-AP

Synonyms

E74 like factor 2, ELF2, ELF2A, EU32, NERF, NERF 1A, NERF 1a,b, NERF 1B, NERF 2, New ETS related factor

Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using ELF2 Polyclonal antibody (12499-1-AP)

    WB analysis of A2780 using 12499-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ELF2 Polyclonal antibody (12499-1-AP)

    WB analysis of human liver using 12499-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ELF2 Polyclonal antibody (12499-1-AP)

    WB analysis of human brain using 12499-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A2780 cells using ELF2 Polyclonal antibody (12499-1-AP)

    IP experiment of A2780 using 12499-1-AP

    IP result of anti-ELF2 (IP:12499-1-AP, 4ug; Detection:12499-1-AP 1:500) with A2780 cells lysate 960ug.

Tested Applications

Positive WB detected inA2780 cells, human brain tissue, human liver tissue
Positive IP detected inA2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12499-1-AP targets ELF2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELF2 fusion protein Ag3170
Full Name E74-like factor 2 (ets domain transcription factor)
Calculated Molecular Weight 533 aa, 57 kDa
Observed Molecular Weight 63-64 kDa, 56-57 kDa
GenBank Accession NumberBC034951
Gene Symbol ELF2
Gene ID (NCBI) 1998
RRIDAB_10642956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.

Protocols

Product Specific Protocols
WB protocol for ELF2 antibody 12499-1-APDownload protocol
IP protocol for ELF2 antibody 12499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB

Front Cell Dev Biol

A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.

Authors - Yue Zhang
  • KD Validated
View Article
humanWB

Cell Biol Int

Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.

Authors - Yunpeng Bai
View Article
mouseWB

Ren Fail

ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.

Authors - Shizhu Yuan
View Article