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ENTPD8 Polyclonal antibody

ENTPD8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18848-1-AP

Synonyms

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ENTPD8 Polyclonal antibody (18848-1-AP)

    WB analysis of HepG2 using 18848-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18848-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18848-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ectonucleoside triphosphate diphosphohydrolase 8
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberNM_198585
Gene Symbol ENTPD8
Gene ID (NCBI) 377841
RRIDAB_10597090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD8, also named as UNQ2492 and PRO5779, belongs to the GDA1/CD39 NTPase family. ENTPD8 is a canalicular ectonucleoside NTPDase which responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. ENTPD8 catalyze the reaction :ATP + 2 H2O = AMP + 2 phosphate.

Protocols

Product Specific Protocols
WB protocol for ENTPD8 antibody 18848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols