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EPS15R Polyclonal antibody

EPS15R Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21243-1-AP

Synonyms

Eps15 related protein, EPS15L1, EPS15R

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPS15R Polyclonal antibody (21243-1-AP)

    WB analysis using 21243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using EPS15R Polyclonal antibody (21243-1-AP)

    WB analysis of MCF-7 using 21243-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using EPS15R Polyclonal antibody (21243-1-AP)

    IF Staining of BxPC-3 using 21243-1-AP

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using 21243-1-AP (EPS15R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, MCF-7 cells, HEK-293 cells, Y79 cells
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21243-1-AP is EPS15R Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPS15R fusion protein Ag15735
Full Name epidermal growth factor receptor pathway substrate 15-like 1
Calculated Molecular Weight 864 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC142716
Gene Symbol EPS15L1
Gene ID (NCBI) 58513
RRIDAB_10733643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EPS15R antibody 21243-1-APDownload protocol
IF protocol for EPS15R antibody 21243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
View Article
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article