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FAM185A Polyclonal antibody

FAM185A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20911-1-AP

Synonyms

FAM185A, Protein FAM185A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAM185A Polyclonal antibody (20911-1-AP)

    WB analysis of HeLa using 20911-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FAM185A Polyclonal antibody (20911-1-AP)

    WB analysis of MCF-7 using 20911-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FAM185A Polyclonal antibody (20911-1-AP)

    WB analysis of HEK-293 using 20911-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using FAM185A Polyclonal antibody (20911-1-AP)

    IHC staining of human hepatocirrhosis using 20911-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using FAM185A Polyclonal antibody (20911-1-AP)

    IHC staining of human hepatocirrhosis using 20911-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using FAM185A Polyclonal antibody (20911-1-AP)

    IHC staining of human spleen using 20911-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using FAM185A Polyclonal antibody (20911-1-AP)

    IHC staining of human spleen using 20911-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20911-1-AP is FAM185A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM185A fusion protein Ag15041
Full Name family with sequence similarity 185, member A
Calculated Molecular Weight 393 aa, 42 kDa
Observed Molecular Weight 30 kDa, 42 kDa
GenBank Accession NumberBC029175
Gene Symbol FAM185A
Gene ID (NCBI) 222234
RRIDAB_10732807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM185A antibody 20911-1-APDownload protocol
IHC protocol for FAM185A antibody 20911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols