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GLYCTK Polyclonal antibody

GLYCTK Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12426-1-AP

Synonyms

glycerate kinase, GLYCTK, HBeAg binding protein 4, HBeAgBP4A, HBEBP2, HBEBP4

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using GLYCTK Polyclonal antibody (12426-1-AP)

    WB analysis of human lung using 12426-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12426-1-AP (GLYCTK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GLYCTK Polyclonal antibody (12426-1-AP)

    WB analysis of human liver using 12426-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12426-1-AP (GLYCTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GLYCTK Polyclonal antibody (12426-1-AP)

    IHC staining of human pancreas cancer using 12426-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12426-1-AP (GLYCTK antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GLYCTK Polyclonal antibody (12426-1-AP)

    IF Staining of HeLa using 12426-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLYCTK antibody (12426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inhuman lung tissue, human liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12426-1-AP targets GLYCTK in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLYCTK fusion protein Ag3097
Full Name glycerate kinase
Calculated Molecular Weight 523 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC021896
Gene Symbol GLYCTK
Gene ID (NCBI) 132158
RRIDAB_2110802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLYCTK antibody 12426-1-APDownload protocol
IHC protocol for GLYCTK antibody 12426-1-APDownload protocol
IF protocol for GLYCTK antibody 12426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols