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GPRIN3-Specific Polyclonal antibody

GPRIN3-Specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20212-1-AP

Synonyms

GPRIN family member 3, GPRIN3, GRIN3, KIAA2027

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    WB analysis of human brain using 20212-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    WB analysis of mouse brain using 20212-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    IF Staining of HepG2 using 20212-1-AP

    Immunofluorescent analysis of HepG2 cells, using GPRIN3 antibody 20212-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20212-1-AP targets GPRIN3-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GPRIN family member 3
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberNM_198281
Gene Symbol GPRIN3
Gene ID (NCBI) 285513
RRIDAB_10666200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPRIN3, also named as KIAA2027, may be involved in neurite outgrowth. The antibody is specific to GPRIN3.

Protocols

Product Specific Protocols
WB protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
IF protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

UBE3D Regulates mRNA 3\'-End Processing and Maintains Adipogenic Potential in 3T3-L1 Cells

Authors - Daniel Heller-Trulli
View Article