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  • Featured Product
  • KD/KO Validated

GSTCD Polyclonal antibody

GSTCD Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17502-1-AP

Synonyms

GSTCD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTCD Polyclonal antibody (17502-1-AP)

    WB analysis using 17502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17502-1-AP (GSTCD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GSTCD Polyclonal antibody (17502-1-AP)

    WB analysis of L02 using 17502-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17502-1-AP (GSTCD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTCD Polyclonal antibody (17502-1-AP)

    IHC staining of human liver using 17502-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17502-1-AP (GSTCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GSTCD Polyclonal antibody (17502-1-AP)

    IHC staining of mouse lung using 17502-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17502-1-AP (GSTCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GSTCD Polyclonal antibody (17502-1-AP)

    IHC staining of mouse lung using 17502-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17502-1-AP (GSTCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GSTCD Polyclonal antibody (17502-1-AP)

    IF Staining of A549 using 17502-1-AP

    Immunofluorescent analysis of A549 cells using 17502-1-AP (GSTCD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, L02 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17502-1-AP targets GSTCD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTCD fusion protein Ag11690
Full Name glutathione S-transferase, C-terminal domain containing
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032942
Gene Symbol GSTCD
Gene ID (NCBI) 79807
RRIDAB_2115908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutathione S-transferase, C-terminal domain-containing protein (GSTCD) is widely expressed in cell types relevant to airway function, including airway smooth muscle cells and epithelial cells.

Protocols

Product Specific Protocols
WB protocol for GSTCD antibody 17502-1-APDownload protocol
IHC protocol for GSTCD antibody 17502-1-APDownload protocol
IF protocol for GSTCD antibody 17502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Respir Res

Defining a role for lung function associated gene GSTCD in cell homeostasis.

Authors - Amanda P Henry
  • KD Validated
View Article
mouseIHC

PLoS One

Exposure to lipopolysaccharide (LPS) reduces contractile response of small airways from GSTCD-/- mice.

Authors - Bo Liu
View Article