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IL-36 Beta/IL-1F8 Monoclonal antibody

IL-36 Beta/IL-1F8 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

10D12B1

Cat no : 60290-1-Ig

Synonyms

FIL1, FIL1 (ETA), FIL1 eta, FIL1H, IL 1 eta, IL 1F8, IL 1H2, IL1 ETA, IL1F8, IL-1F8, IL1H2, IL-36 beta, IL-36 Beta/IL-1F8, IL36B, Interleukin 1 eta, Interleukin 1 family member 8, Interleukin 1 homolog 2, Interleukin-36 beta

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    WB analysis of Recombinant protein using 60290-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60290-1-Ig (IL1F8 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IHC staining of human tonsillitis using 60290-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IHC staining of human tonsillitis using 60290-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IF Staining of human tonsillitis using 60290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IF Staining of human tonsillitis using 60290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60290-1-Ig targets IL-36 Beta/IL-1F8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-36 Beta/IL-1F8 fusion protein Ag12675
Full Name interleukin 1 family, member 8 (eta)
Calculated Molecular Weight 157 aa, 18 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC101833
Gene Symbol IL1F8
Gene ID (NCBI) 27177
RRIDAB_2881406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
IHC protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
IF protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols