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INSC Polyclonal antibody

INSC Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 20973-1-AP

Synonyms

INSC, Protein inscuteable homolog

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse liver using 20973-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse liver using 20973-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse kidney using 20973-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse kidney using 20973-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human kidney using 20973-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human kidney using 20973-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human liver using 20973-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human liver using 20973-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INSC Polyclonal antibody (20973-1-AP)

    IF Staining of HeLa using 20973-1-AP

    Immunofluorescent analysis of HeLa cells using 20973-1-AP (INSC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ChIPSee 1 publications below

Product Information

20973-1-AP targets INSC in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inscuteable homolog (Drosophila)
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberNM_001031853
Gene Symbol INSC
Gene ID (NCBI) 387755
RRIDAB_10951111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC.

Protocols

Product Specific Protocols
WB protocol for INSC antibody 20973-1-APDownload protocol
IHC protocol for INSC antibody 20973-1-APDownload protocol
IF protocol for INSC antibody 20973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ChIP

Cell Rep

The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus.

Authors - Bhavana Gupta
View Article