INTS8 Polyclonal antibody
INTS8 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18802-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
18802-1-AP targets INTS8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | integrator complex subunit 8 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 90-115 kDa |
| GenBank Accession Number | BC136754 |
| Gene Symbol | INTS8 |
| Gene ID (NCBI) | 55656 |
| RRID | AB_10597250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INTS8, also named as C8orf52, is a Component of the Integrator complex which is a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. INTS8 is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INTS8 antibody 18802-1-AP | Download protocol |
| IF protocol for INTS8 antibody 18802-1-AP | Download protocol |
| IP protocol for INTS8 antibody 18802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
J Exp Clin Cancer Res Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma. | ||
Cancer Manag Res INTS8 accelerates the epithelial-to-mesenchymal transition in hepatocellular carcinoma by upregulating the TGF-β signaling pathway. | ||
Cell Rep A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder |
