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ISOC2 Polyclonal antibody

ISOC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27082-1-AP

Synonyms

ISOC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ISOC2 Polyclonal antibody (27082-1-AP)

    WB analysis using 27082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ISOC2 Polyclonal antibody (27082-1-AP)

    WB analysis of LNCaP using 27082-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    WB analysis of mouse kidney using 27082-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    WB analysis of mouse liver using 27082-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    IHC staining of human liver using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ISOC2 Polyclonal antibody (27082-1-AP)

    IHC staining of human liver using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inLNCaP cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27082-1-AP targets ISOC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISOC2 fusion protein Ag23936
Full Name isochorismatase domain containing 2
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017344
Gene Symbol ISOC2
Gene ID (NCBI) 79763
RRIDAB_2880748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ISOC2 antibody 27082-1-APDownload protocol
IHC protocol for ISOC2 antibody 27082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols