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KANK2 Polyclonal antibody

KANK2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20546-1-AP

Synonyms

ANKRD25, KANK2, KIAA1518, MXRA3, SIP, SRC 1 interacting protein, SRC1 interacting protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human kidney using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human kidney using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KANK2 Polyclonal antibody (20546-1-AP)

    IF Staining of HeLa using 20546-1-AP

    Immunofluorescent analysis of Hela cells, using KANK2 antibody 20546-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20546-1-AP targets KANK2 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name KN motif and ankyrin repeat domains 2
Calculated Molecular Weight 91 kDa
GenBank Accession NumberNM_001136191
Gene Symbol KANK2
Gene ID (NCBI) 25959
RRIDAB_10694286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.

Protocols

Product Specific Protocols
IHC protocol for KANK2 antibody 20546-1-APDownload protocol
IF protocol for KANK2 antibody 20546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols