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KBTBD10 Polyclonal antibody

KBTBD10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25577-1-AP

Synonyms

KBTBD10, Kel like protein 23, Kelch related protein 1, Kelch-like protein 41, KLH41, KLHL41, KRP1, SARCOSIN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KBTBD10 Polyclonal antibody (25577-1-AP)

    WB analysis using 25577-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25577-1-AP (KBTBD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using KBTBD10 Polyclonal antibody (25577-1-AP)

    IHC staining of human skeletal muscle using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KBTBD10 Polyclonal antibody (25577-1-AP)

    IHC staining of human heart using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using KBTBD10 Polyclonal antibody (25577-1-AP)

    IHC staining of human skeletal muscle using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KBTBD10 Polyclonal antibody (25577-1-AP)

    IHC staining of human heart using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25577-1-AP targets KBTBD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KBTBD10 fusion protein Ag22311
Full Name kelch repeat and BTB (POZ) domain containing 10
Calculated Molecular Weight 606 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006534
Gene Symbol KBTBD10
Gene ID (NCBI) 10324
RRIDAB_2880137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KBTBD10, also named as KBTBD10 or KRP1, is a 606 amino acid protein, which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and five Kelch repeats. KBTBD10 localizes in the cytoplasm and is expressed in Sarcomeric muscle. KBTBD10 is involved in skeletal muscle development and differentiation. KBTBD10 regulates proliferation and differentiation of myoblasts and plays a role in myofibril assembly by promoting lateral fusion of adjacent thin fibrils into mature, wide myofibrils.

Protocols

Product Specific Protocols
WB protocol for KBTBD10 antibody 25577-1-APDownload protocol
IHC protocol for KBTBD10 antibody 25577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols