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  • Featured Product
  • KD/KO Validated

KRCC1 Polyclonal antibody

KRCC1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16916-1-AP

Synonyms

BM 044, CHBP2, KRCC1, lysine rich coiled coil 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRCC1 Polyclonal antibody (16916-1-AP)

    WB analysis using 16916-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16916-1-AP (KRCC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KRCC1 Polyclonal antibody (16916-1-AP)

    WB analysis of MCF-7 using 16916-1-AP

    WB result of KRCC1 antibody (16916-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRCC1 transfected MCF-7 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KRCC1 Polyclonal antibody (16916-1-AP)

    IF Staining of HepG2 using 16916-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16916-1-AP (KRCC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16916-1-AP targets KRCC1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRCC1 fusion protein Ag10157
Full Name lysine-rich coiled-coil 1
Calculated Molecular Weight 187 aa, 22 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC107580
Gene Symbol KRCC1
Gene ID (NCBI) 51315
RRIDAB_2878331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KRCC1 antibody 16916-1-APDownload protocol
IF protocol for KRCC1 antibody 16916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

KRCC1: A potential therapeutic target in ovarian cancer.

Authors - Shailendra Kumar Dhar Dwivedi
View Article
humanWB,IF

Nucleic Acids Res

KRCC1, a modulator of the DNA damage response

Authors - Fiifi Neizer-Ashun
  • KD Validated
View Article