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LAMR1,RPSA Monoclonal antibody

LAMR1,RPSA Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A9A1

Cat no : 67324-1-Ig

Synonyms

37/67 kDa laminin receptor, 37LRP, 40S ribosomal protein SA, 67 kDa laminin receptor, 67LR, LAMBR, Laminin receptor, Laminin receptor 1, LamR, LAMR1, LAMR1,RPSA, LBP/p40, LRP, LRP/LR, NEM/1CHD4, p40, ribosomal protein SA, RPSA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMR1,RPSA Monoclonal antibody (67324-1-Ig)

    WB analysis using 67324-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LAMR1,RPSA Monoclonal antibody (67324-1-Ig)

    IHC staining of human lung cancer using 67324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LAMR1,RPSA Monoclonal antibody (67324-1-Ig)

    IHC staining of human lung cancer using 67324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LAMR1,RPSA Monoclonal antibody (67324-1-Ig)

    IF Staining of HepG2 using 67324-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1,RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, COLO 320 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67324-1-Ig targets LAMR1,RPSA in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen LAMR1,RPSA fusion protein Ag6190
Full Name ribosomal protein SA
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC050688
Gene Symbol RPSA
Gene ID (NCBI) 3921
RRIDAB_2882584
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.

Protocols

Product Specific Protocols
WB protocol for LAMR1,RPSA antibody 67324-1-IgDownload protocol
IHC protocol for LAMR1,RPSA antibody 67324-1-IgDownload protocol
IF protocol for LAMR1,RPSA antibody 67324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization

Authors - Lele Li
View Article