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LAS1L Polyclonal antibody

LAS1L Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16010-1-AP

Synonyms

LAS1 like (S. cerevisiae), LAS1L, Protein LAS1 homolog

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LAS1L Polyclonal antibody (16010-1-AP)

    WB analysis using 16010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16010-1-AP (LAS1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using LAS1L Polyclonal antibody (16010-1-AP)

    IP experiment of HEK-293 using 16010-1-AP

    IP result of anti-LAS1L (IP:16010-1-AP, 4ug; Detection:16010-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LAS1L Polyclonal antibody (16010-1-AP)

    IF Staining of HepG2 using 16010-1-AP

    Immunofluorescent analysis of HepG2 cells using 16010-1-AP (LAS1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16010-1-AP targets LAS1L in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAS1L fusion protein Ag8732
Full Name LAS1-like (S. cerevisiae)
Calculated Molecular Weight 734 aa, 83 kDa
Observed Molecular Weight 83 kDa, 70-75 kDa
GenBank Accession NumberBC014545
Gene Symbol LAS1L
Gene ID (NCBI) 81887
RRIDAB_2132810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.

Protocols

Product Specific Protocols
WB protocol for LAS1L antibody 16010-1-APDownload protocol
IF protocol for LAS1L antibody 16010-1-APDownload protocol
IP protocol for LAS1L antibody 16010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.

Authors - Fanis Pavlos P
View Article
humanWB

Breast Cancer Res Treat

Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34

Authors - Kristin A Altwegg
View Article
humanWB

Mol Oncol

PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Authors - Xue Yang
View Article