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LCE1B Polyclonal antibody

LCE1B Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21771-1-AP

Synonyms

late cornified envelope 1B, Late envelope protein 2, LCE1B, LEP2, SPRL2A

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using LCE1B Polyclonal antibody (21771-1-AP)

    WB analysis of A375 using 21771-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human oesophagus using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human oesophagus using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using LCE1B Polyclonal antibody (21771-1-AP)

    IF Staining of A375 using 21771-1-AP

    Immunofluorescent analysis of A375 cells using 21771-1-AP (LCE1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21771-1-AP is LCE1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCE1B fusion protein Ag16442
Full Name late cornified envelope 1B
Calculated Molecular Weight 118 aa, 12 kDa
Observed Molecular Weight 10-12 kDa
GenBank Accession NumberBC104232
Gene Symbol LCE1B
Gene ID (NCBI) 353132
RRIDAB_10858482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).

Protocols

Product Specific Protocols
WB protocol for LCE1B antibody 21771-1-APDownload protocol
IHC protocol for LCE1B antibody 21771-1-APDownload protocol
IF protocol for LCE1B antibody 21771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols