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LGR6 Polyclonal antibody
LGR6 Polyclonal Antibody for WB, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17658-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, mouse testis tissue, human brain tissue |
| Positive IHC detected in | human testis tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
17658-1-AP targets LGR6 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LGR6 fusion protein Ag11909 |
| Full Name | leucine-rich repeat-containing G protein-coupled receptor 6 |
| Calculated Molecular Weight | 915 aa, 99 kDa |
| Observed Molecular Weight | 104 kDa |
| GenBank Accession Number | BC047905 |
| Gene Symbol | LGR6 |
| Gene ID (NCBI) | 59352 |
| RRID | AB_2135163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LGR6 antibody 17658-1-AP | Download protocol |
| IHC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| FC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.
| ||
Mol Ther Oncolytics Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer. | ||
Proc Natl Acad Sci U S A The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation | ||
Aging (Albany NY) Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marc (Verified Customer) (02-14-2023) | Used this antibody on immunofluorescence, but the expression of LGR6 in the spinal cord is low so we had to perform an immunofluorescence with tyramide amplification. With tyramide amplification the result is satisfactory.
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