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LMF2 Polyclonal antibody

LMF2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16249-1-AP

Synonyms

lipase maturation factor 2, LMF2, TMEM112B, TMEM153, Transmembrane protein 112B, Transmembrane protein 153

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LMF2 Polyclonal antibody (16249-1-AP)

    WB analysis of HeLa using 16249-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16249-1-AP (LMF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LMF2 Polyclonal antibody (16249-1-AP)

    WB analysis of human brain using 16249-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16249-1-AP (LMF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LMF2 Polyclonal antibody (16249-1-AP)

    IHC staining of human kidney using 16249-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LMF2 Polyclonal antibody (16249-1-AP)

    IHC staining of human kidney using 16249-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LMF2 Polyclonal antibody (16249-1-AP)

    IF Staining of MCF-7 using 16249-1-AP

    Immunofluorescent analysis of MCF-7 cells, using 16249-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16249-1-AP targets LMF2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMF2 fusion protein Ag9208
Full Name lipase maturation factor 2
Calculated Molecular Weight 682 aa, 77 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014652
Gene Symbol LMF2
Gene ID (NCBI) 91289
RRIDAB_2136142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LMF2 antibody 16249-1-APDownload protocol
IHC protocol for LMF2 antibody 16249-1-APDownload protocol
IF protocol for LMF2 antibody 16249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols