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  • Featured Product
  • KD/KO Validated

LOH12CR1 Polyclonal antibody

LOH12CR1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17169-1-AP

Synonyms

LOH12CR1, LOH1CR12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LOH12CR1 Polyclonal antibody (17169-1-AP)

    WB analysis using 17169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17169-1-AP (LOH12CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LOH12CR1 Polyclonal antibody (17169-1-AP)

    IHC staining of human lung cancer using 17169-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LOH12CR1 Polyclonal antibody (17169-1-AP)

    IHC staining of human lung cancer using 17169-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LOH12CR1 Polyclonal antibody (17169-1-AP)

    IHC staining of human liver cancer using 17169-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LOH12CR1 Polyclonal antibody (17169-1-AP)

    IHC staining of human liver cancer using 17169-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LOH12CR1 Polyclonal antibody (17169-1-AP)

    IF Staining of U2OS using 17169-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17169-1-AP targets LOH12CR1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LOH12CR1 fusion protein Ag10695
Full Name loss of heterozygosity, 12, chromosomal region 1
Calculated Molecular Weight 196 aa, 22 kDa
Observed Molecular Weight 17-22 kDa
GenBank Accession NumberBC013668
Gene Symbol LOH12CR1
Gene ID (NCBI) 118426
RRIDAB_2137150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LOH12CR1, also named as BORCS5, is part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. It may indirectly play a role in cell spreading and motility. LOH12CR1 has two isoforms with MW 17 kDa and 22 kDa.

Protocols

Product Specific Protocols
WB protocol for LOH12CR1 antibody 17169-1-APDownload protocol
IHC protocol for LOH12CR1 antibody 17169-1-APDownload protocol
IF protocol for LOH12CR1 antibody 17169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Dev Cell

BORC, a Multisubunit Complex that Regulates Lysosome Positioning.

Authors - Jing Pu
View Article
humanWB

Autophagy

HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.

Authors - Maryline Santerre
View Article
mouseWB

Cell Rep

Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.

Authors - Raffaella De Pace
  • KO Validated
View Article
humanWB

Cell Genom

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders

Authors - Greta Pintacuda
View Article