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LRMP Polyclonal antibody

LRMP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19498-1-AP

Synonyms

JAW1, LRMP, Protein Jaw1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Raji using 19498-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Jurkat using 19498-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRMP Polyclonal antibody (19498-1-AP)

    IF Staining of HepG2 using 19498-1-AP

    Immunofluorescent analysis of HepG2 cells, using LRMP antibody 19498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19498-1-AP targets LRMP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lymphoid-restricted membrane protein
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberNM_006152
Gene Symbol LRMP
Gene ID (NCBI) 4033
RRIDAB_10640430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for LRMP antibody 19498-1-APDownload protocol
IHC protocol for LRMP antibody 19498-1-APDownload protocol
IF protocol for LRMP antibody 19498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols