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MARVELD3 Polyclonal antibody

MARVELD3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25667-1-AP

Synonyms

MARVD3, MARVEL domain containing 3, MARVELD3, MRVLDC3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using MARVELD3 Polyclonal antibody (25667-1-AP)

    WB analysis of COLO 320 using 25667-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25667-1-AP (MARVELD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MARVELD3 Polyclonal antibody (25667-1-AP)

    IHC staining of human kidney using 25667-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MARVELD3 Polyclonal antibody (25667-1-AP)

    IHC staining of human kidney using 25667-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using MARVELD3 Polyclonal antibody (25667-1-AP)

    IF Staining of Caco-2 using 25667-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using MARVELD3 antibody (25667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 25667-1-AP is MARVELD3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARVELD3 fusion protein Ag22492
Full Name MARVEL domain containing 3
Calculated Molecular Weight 401 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC013376
Gene Symbol MARVELD3
Gene ID (NCBI) 91862
RRIDAB_2880184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).

Protocols

Product Specific Protocols
WB protocol for MARVELD3 antibody 25667-1-APDownload protocol
IHC protocol for MARVELD3 antibody 25667-1-APDownload protocol
IF protocol for MARVELD3 antibody 25667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers

Authors - Ha-Young Park
View Article
humanWB

FEBS Open Bio

Hesperidin enhances intestinal barrier function in Caco-2 cell monolayers via AMPK-mediated tight junction-related proteins

Authors - Ha-Young Park
View Article