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MCM4 Monoclonal antibody

MCM4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H2A1

Cat no : 67103-1-Ig

Synonyms

CDC21, CDC21 homolog, CDC54, hCdc21, MCM4, P1 CDC21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis using 67103-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis of HSC-T6 using 67103-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis of NIH/3T3 using 67103-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A431 cells, HL-60 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67103-1-Ig targets MCM4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCM4 fusion protein Ag28642
Full Name minichromosome maintenance complex component 4
Calculated Molecular Weight 863 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC031061
Gene Symbol MCM4
Gene ID (NCBI) 4173
RRIDAB_2882408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Protocols

Product Specific Protocols
WB protocol for MCM4 antibody 67103-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols