MDM1 Polyclonal antibody

MDM1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17575-1-AP

Synonyms

MDM1, Nuclear protein MDM1



Tested Applications

Positive WB detected inmouse retina tissue, Raji cells, rat retina tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17575-1-AP targets MDM1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDM1 fusion protein Ag10514
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC028355
Gene Symbol MDM1
Gene ID (NCBI) 56890
RRIDAB_2281929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MDM1 antibody 17575-1-APDownload protocol
IHC protocol for MDM1 antibody 17575-1-APDownload protocol
IF protocol for MDM1 antibody 17575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cells

Authors - Youlim Son
  • KO Validated