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METTL5 Polyclonal antibody
METTL5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, RIP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16791-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IHC | See 1 publications below |
| RIP | See 1 publications below |
Product Information
16791-1-AP targets METTL5 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | METTL5 fusion protein Ag10232 |
| Full Name | methyltransferase like 5 |
| Calculated Molecular Weight | 209 aa, 24 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC000921 |
| Gene Symbol | METTL5 |
| Gene ID (NCBI) | 29081 |
| RRID | AB_2142051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IHC protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IF protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IP protocol for METTL5 antibody 16791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.
| ||
J Biol Chem The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
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Front Cardiovasc Med Loss of m6A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression. | ||
Bioengineered The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.
| ||
Bioengineered Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA. | ||
Cell Rep Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.
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