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MLL5 Polyclonal antibody

MLL5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14540-1-AP

Synonyms

HDCMC04P, KMT2E, Lysine N methyltransferase 2E, MLL5

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using MLL5 Polyclonal antibody (14540-1-AP)

    WB analysis of HL-60 using 14540-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MLL5 Polyclonal antibody (14540-1-AP)

    WB analysis using 14540-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MLL5 Polyclonal antibody (14540-1-AP)

    IHC staining of human pancreas cancer using 14540-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MLL5 Polyclonal antibody (14540-1-AP)

    IHC staining of human pancreas cancer using 14540-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using MLL5 Polyclonal antibody (14540-1-AP)

    IF Staining of K-562 using 14540-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using MLL5 antibody (14540-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, Raji cells, mouse retina tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14540-1-AP targets MLL5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLL5 fusion protein Ag6044
Full Name myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
Calculated Molecular Weight 205 kDa
Observed Molecular Weight 100 kDa, 70 kDa
GenBank Accession NumberBC062583
Gene Symbol MLL5
Gene ID (NCBI) 55904
RRIDAB_2878065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MLL5 antibody 14540-1-APDownload protocol
IHC protocol for MLL5 antibody 14540-1-APDownload protocol
IF protocol for MLL5 antibody 14540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell

SARS-CoV-2 RNA stabilizes host mRNAs to elicit immunopathogenesis

Authors - Hailian Zhao
View Article