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MPHOSPH6 Polyclonal antibody

MPHOSPH6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10695-1-AP

Synonyms

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MCF-7 using 10695-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MCF-7 using 10695-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of HeLa using 10695-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of HeLa using 10695-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MDA-MB-453s using 10695-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    IP experiment of HeLa using 10695-1-AP

    IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    IHC staining of human breast cancer using 10695-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, MCF-7 cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10695-1-AP targets MPHOSPH6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPHOSPH6 fusion protein Ag1080
Full Name M-phase phosphoprotein 6
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC005242
Gene Symbol MPHOSPH6
Gene ID (NCBI) 10200
RRIDAB_2282161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
IHC protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
IP protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Prolif

The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.

Authors - Rui-Rui Peng
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody that specifically recognise Mphosph6

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:10695-1-AP)
FH

Ralf (Verified Customer) (08-04-2022)

10µg of the total mouse protein were loaded on the gel. Antibodies also stain the ladder a bit.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in (Cat no:10695-1-AP)