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MRPL28 Polyclonal antibody

MRPL28 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21604-1-AP

Synonyms

L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL28 Polyclonal antibody (21604-1-AP)

    WB analysis using 21604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21604-1-AP (MRPL28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MRPL28 Polyclonal antibody (21604-1-AP)

    IP experiment of mouse brain using 21604-1-AP

    IP result of anti-MRPL28 (IP:21604-1-AP, 4ug; Detection:21604-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL28 Polyclonal antibody (21604-1-AP)

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL28 Polyclonal antibody (21604-1-AP)

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL28 Polyclonal antibody (21604-1-AP)

    IHC staining of human testis using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL28 Polyclonal antibody (21604-1-AP)

    IHC staining of human testis using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL28 Polyclonal antibody (21604-1-AP)

    IF Staining of HeLa using 21604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL28 antibody (21604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, JAR cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21604-1-AP is MRPL28 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL28 fusion protein Ag16224
Full Name mitochondrial ribosomal protein L28
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC000990
Gene Symbol MRPL28
Gene ID (NCBI) 10573
RRIDAB_10859785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL28 antibody 21604-1-APDownload protocol
IHC protocol for MRPL28 antibody 21604-1-APDownload protocol
IF protocol for MRPL28 antibody 21604-1-APDownload protocol
IP protocol for MRPL28 antibody 21604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article
humanWB

J Ethnopharmacol

Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma - An in vitro study

Authors - Zhishan Wu
View Article