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MRPL46 Polyclonal antibody

MRPL46 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16611-1-AP

Synonyms

C15orf4, L46mt, LIECG2, MRP L46, MRPL46, P2ECSL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL46 Polyclonal antibody (16611-1-AP)

    WB analysis using 16611-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16611-1-AP (MRPL46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MRPL46 Polyclonal antibody (16611-1-AP)

    IHC staining of human heart using 16611-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MRPL46 Polyclonal antibody (16611-1-AP)

    IHC staining of human heart using 16611-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL46 Polyclonal antibody (16611-1-AP)

    IHC staining of human kidney using 16611-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL46 Polyclonal antibody (16611-1-AP)

    IHC staining of human kidney using 16611-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL46 Polyclonal antibody (16611-1-AP)

    IF Staining of HepG2 using 16611-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16611-1-AP targets MRPL46 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL46 fusion protein Ag9837
Full Name mitochondrial ribosomal protein L46
Calculated Molecular Weight 279 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC017883
Gene Symbol MRPL46
Gene ID (NCBI) 26589
RRIDAB_2146074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL46 antibody 16611-1-APDownload protocol
IHC protocol for MRPL46 antibody 16611-1-APDownload protocol
IF protocol for MRPL46 antibody 16611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism

Authors - Ying Shu
View Article