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MRPS21 Polyclonal antibody

MRPS21 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16359-1-AP

Synonyms

MDS016, MRP S21, MRPS21, RPMS21, S21mt

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pancreas tissue using MRPS21 Polyclonal antibody (16359-1-AP)

    IHC staining of human pancreas using 16359-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16359-1-AP (MRPS21 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MRPS21 Polyclonal antibody (16359-1-AP)

    IHC staining of human pancreas using 16359-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16359-1-AP (MRPS21 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPS21 Polyclonal antibody (16359-1-AP)

    IF Staining of HepG2 using 16359-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16359-1-AP (MRPS21 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16359-1-AP targets MRPS21 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS21 fusion protein Ag8343
Full Name mitochondrial ribosomal protein S21
Calculated Molecular Weight 87 aa, 11 kDa
GenBank Accession NumberBC004566
Gene Symbol MRPS21
Gene ID (NCBI) 54460
RRIDAB_2180349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MRPS21 antibody 16359-1-APDownload protocol
IF protocol for MRPS21 antibody 16359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols