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MTRF1L Polyclonal antibody

MTRF1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16694-1-AP

Synonyms

MRF1L, mtRF1a, MTRF1L

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HEK-293 using 16694-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HEK-293 using 16694-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HEK-293 using 16694-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HepG2 using 16694-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HeLa using 16694-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HEK-293 using 16694-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MTRF1L Polyclonal antibody (16694-1-AP)

    WB analysis of HeLa using 16694-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16694-1-AP targets MTRF1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTRF1L fusion protein Ag10081
Full Name mitochondrial translational release factor 1-like
Calculated Molecular Weight 380 aa, 44 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC011873
Gene Symbol MTRF1L
Gene ID (NCBI) 54516
RRIDAB_2878301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTRF1L, also named as Peptide chain release factor 1-like, mitochondrial, is a 380 amino acid protein, which belongs to the prokaryotic/mitochondrial release factor family and locates in the mitochondrial. MTRF1L directs the termination of translation in response to the peptide chain termination codons UAA and UAG. MTRF1L is expressed in skeletal muscle.

Protocols

Product Specific Protocols
WB protocol for MTRF1L antibody 16694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Authors - Takumi Yokokawa
View Article
mouseWB

Physiol Rep

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Authors - Takumi Yokokawa
View Article
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
View Article