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Myoferlin Polyclonal antibody

Myoferlin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19548-1-AP

Synonyms

Fer 1 like protein 3, FER1L3, KIAA1207, MYOF, Myoferlin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Myoferlin Polyclonal antibody (19548-1-AP)

    WB analysis using 19548-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Myoferlin Polyclonal antibody (19548-1-AP)

    WB analysis of A549 using 19548-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Myoferlin Polyclonal antibody (19548-1-AP)

    WB analysis of human placenta using 19548-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Myoferlin Polyclonal antibody (19548-1-AP)

    IHC staining of human placenta using 19548-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Myoferlin Polyclonal antibody (19548-1-AP)

    IHC staining of human placenta using 19548-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Myoferlin Polyclonal antibody (19548-1-AP)

    IF Staining of HeLa using 19548-1-AP

    Immunofluorescent analysis of Hela cells, using MYOF antibody 19548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HeLa cells using Myoferlin Polyclonal antibody (19548-1-AP)

    FC experiment of HeLa using 19548-1-AP

    1X10^6 HeLa cells were stained with 0.2ug Myoferlin antibody (19548-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inA549 cells, human placenta tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19548-1-AP targets Myoferlin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myoferlin
Calculated Molecular Weight 235 kDa
Observed Molecular Weight 235-250 kDa
GenBank Accession NumberNM_133337
Gene Symbol MYOF
Gene ID (NCBI) 26509
RRIDAB_10642558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.

Protocols

Product Specific Protocols
WB protocol for Myoferlin antibody 19548-1-APDownload protocol
IHC protocol for Myoferlin antibody 19548-1-APDownload protocol
IF protocol for Myoferlin antibody 19548-1-APDownload protocol
FC protocol for Myoferlin antibody 19548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteomics

Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

Authors - Yanqing Zhou
View Article
humanWB

Eur J Med Chem

Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo

Authors - Xuwen Chen
View Article