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SLC13A3 Polyclonal antibody

SLC13A3 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26184-1-AP

Synonyms

hNaDC3, NaDC 3, NADC3, SDCT2, SLC13A3

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SLC13A3 Polyclonal antibody (26184-1-AP)

    IP experiment of mouse kidney using 26184-1-AP

    IP result of anti-SLC13A3 (IP:26184-1-AP, 4ug; Detection:26184-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC13A3 Polyclonal antibody (26184-1-AP)

    IHC staining of human kidney using 26184-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC13A3 Polyclonal antibody (26184-1-AP)

    IHC staining of human kidney using 26184-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC13A3 Polyclonal antibody (26184-1-AP)

    IHC staining of human kidney using 26184-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC13A3 Polyclonal antibody (26184-1-AP)

    IHC staining of human kidney using 26184-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26184-1-AP targets SLC13A3 in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC13A3 fusion protein Ag24182
Full Name solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
Calculated Molecular Weight 602 aa, 67 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC035966
Gene Symbol SLC13A3
Gene ID (NCBI) 64849
RRIDAB_2868533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC13A3, also named NADC3 and SDCT2, belongs to the SLC13A/DASS transporter (TC 2.A.47) family and NADC subfamily. SLC13A3 encodes the plasma membrane Na+ /Dicarboxylate Cotransporter 3 (NaDC3), which imports inside the cell four to six carbon dicarboxylates as well as N-acetylaspartate (NAA). SLC13A3 is mainly expressed in kidney, but also in brain, liver, placenta and eye (PMID: 30635937).

Protocols

Product Specific Protocols
IHC protocol for SLC13A3 antibody 26184-1-APDownload protocol
IP protocol for SLC13A3 antibody 26184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols